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goat anti human tf polyclonal antibody  (Bethyl)


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    Structured Review

    Bethyl goat anti human tf polyclonal antibody
    Goat Anti Human Tf Polyclonal Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human tf polyclonal antibody/product/Bethyl
    Average 93 stars, based on 36 article reviews
    goat anti human tf polyclonal antibody - by Bioz Stars, 2026-02
    93/100 stars

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    Endosomal distribution of Lrrn1. Immunocytochemical analysis of the endosomal uptake of FLAG-Lrrn1 in HeLa cells. (a, d, g, j) Plasma membrane surface-exposed FLAG-Lrrn1 protein detected with an anti-FLAG antibody. (b, c) Colocalisation with total cellular pool of Lrrn1 detected with the GST-IC antiserum. (e, f) Colocalisation with EEA1 (arrowheads in inset in (f)) shows that a significant pool of Lrrn1 cycles through the early endosomes. (h, i) Little overlap with <t>transferrin</t> receptor immunostaining suggests that Lrrn1 does not partition within recycling endosomes. (k-m) A very small proportion of endosomes were found to co-label with FLAG-Lrrn1/EGF ((m), arrowhead in inset). Scale bars = 200 μm except inset in (f) = 50μm.
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    Endosomal distribution of Lrrn1. Immunocytochemical analysis of the endosomal uptake of FLAG-Lrrn1 in HeLa cells. (a, d, g, j) Plasma membrane surface-exposed FLAG-Lrrn1 protein detected with an anti-FLAG antibody. (b, c) Colocalisation with total cellular pool of Lrrn1 detected with the GST-IC antiserum. (e, f) Colocalisation with EEA1 (arrowheads in inset in (f)) shows that a significant pool of Lrrn1 cycles through the early endosomes. (h, i) Little overlap with <t>transferrin</t> receptor immunostaining suggests that Lrrn1 does not partition within recycling endosomes. (k-m) A very small proportion of endosomes were found to co-label with FLAG-Lrrn1/EGF ((m), arrowhead in inset). Scale bars = 200 μm except inset in (f) = 50μm.
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    . A , Western blotting confirms equal cross-reactivity of <t>polyclonal</t> rabbit anti-human RBP4 antibodies for dog and human RBP4. Samples (65 ng) of heterologously-expressed affinity-purified preparations of histidine-tagged dog (lane 1) and human (lane 2) RBP4 were probed with the antibodies. B , The representative immunoblot comparing RBP4 levels in 10 μl plasma samples from three dogs (lanes 2–4) and three cynomolgus monkeys (lanes 5–7). Human plasma sample (lane 1) was loaded as a reference control. <t>Transferrin</t> antibody was used to re-probe the Western blot to confirm equal loading of plasma samples (lower panel). C , The histogram representing pixel volumes of RBP4 bands. The bars represent pixel volume means ± S.D. of the scanned bands on the immunoblot in arbitrary units.
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    Agilent technologies polyclonal rabbit anti-human transferrin antibodies
    . A , Western blotting confirms equal cross-reactivity of <t>polyclonal</t> rabbit anti-human RBP4 antibodies for dog and human RBP4. Samples (65 ng) of heterologously-expressed affinity-purified preparations of histidine-tagged dog (lane 1) and human (lane 2) RBP4 were probed with the antibodies. B , The representative immunoblot comparing RBP4 levels in 10 μl plasma samples from three dogs (lanes 2–4) and three cynomolgus monkeys (lanes 5–7). Human plasma sample (lane 1) was loaded as a reference control. <t>Transferrin</t> antibody was used to re-probe the Western blot to confirm equal loading of plasma samples (lower panel). C , The histogram representing pixel volumes of RBP4 bands. The bars represent pixel volume means ± S.D. of the scanned bands on the immunoblot in arbitrary units.
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    Endosomal distribution of Lrrn1. Immunocytochemical analysis of the endosomal uptake of FLAG-Lrrn1 in HeLa cells. (a, d, g, j) Plasma membrane surface-exposed FLAG-Lrrn1 protein detected with an anti-FLAG antibody. (b, c) Colocalisation with total cellular pool of Lrrn1 detected with the GST-IC antiserum. (e, f) Colocalisation with EEA1 (arrowheads in inset in (f)) shows that a significant pool of Lrrn1 cycles through the early endosomes. (h, i) Little overlap with transferrin receptor immunostaining suggests that Lrrn1 does not partition within recycling endosomes. (k-m) A very small proportion of endosomes were found to co-label with FLAG-Lrrn1/EGF ((m), arrowhead in inset). Scale bars = 200 μm except inset in (f) = 50μm.

    Journal: Neural Development

    Article Title: Analysis of Lrrn1 expression and its relationship to neuromeric boundaries during chick neural development

    doi: 10.1186/1749-8104-2-22

    Figure Lengend Snippet: Endosomal distribution of Lrrn1. Immunocytochemical analysis of the endosomal uptake of FLAG-Lrrn1 in HeLa cells. (a, d, g, j) Plasma membrane surface-exposed FLAG-Lrrn1 protein detected with an anti-FLAG antibody. (b, c) Colocalisation with total cellular pool of Lrrn1 detected with the GST-IC antiserum. (e, f) Colocalisation with EEA1 (arrowheads in inset in (f)) shows that a significant pool of Lrrn1 cycles through the early endosomes. (h, i) Little overlap with transferrin receptor immunostaining suggests that Lrrn1 does not partition within recycling endosomes. (k-m) A very small proportion of endosomes were found to co-label with FLAG-Lrrn1/EGF ((m), arrowhead in inset). Scale bars = 200 μm except inset in (f) = 50μm.

    Article Snippet: The antibody against EEA1 (rabbit polyclonal antiserum 1:1,500) was a kind gift of Dr Mario Zerial (EMBL, Heidelberg, Germany) and the anti-human transferrin receptor (rabbit polyclonal, 1:250) was from Chemicon.

    Techniques: Immunostaining

    . A , Western blotting confirms equal cross-reactivity of polyclonal rabbit anti-human RBP4 antibodies for dog and human RBP4. Samples (65 ng) of heterologously-expressed affinity-purified preparations of histidine-tagged dog (lane 1) and human (lane 2) RBP4 were probed with the antibodies. B , The representative immunoblot comparing RBP4 levels in 10 μl plasma samples from three dogs (lanes 2–4) and three cynomolgus monkeys (lanes 5–7). Human plasma sample (lane 1) was loaded as a reference control. Transferrin antibody was used to re-probe the Western blot to confirm equal loading of plasma samples (lower panel). C , The histogram representing pixel volumes of RBP4 bands. The bars represent pixel volume means ± S.D. of the scanned bands on the immunoblot in arbitrary units.

    Journal: PLoS ONE

    Article Title: Comparative pharmacokinetics and pharmacodynamics of the advanced Retinol-Binding Protein 4 antagonist in dog and cynomolgus monkey

    doi: 10.1371/journal.pone.0228291

    Figure Lengend Snippet: . A , Western blotting confirms equal cross-reactivity of polyclonal rabbit anti-human RBP4 antibodies for dog and human RBP4. Samples (65 ng) of heterologously-expressed affinity-purified preparations of histidine-tagged dog (lane 1) and human (lane 2) RBP4 were probed with the antibodies. B , The representative immunoblot comparing RBP4 levels in 10 μl plasma samples from three dogs (lanes 2–4) and three cynomolgus monkeys (lanes 5–7). Human plasma sample (lane 1) was loaded as a reference control. Transferrin antibody was used to re-probe the Western blot to confirm equal loading of plasma samples (lower panel). C , The histogram representing pixel volumes of RBP4 bands. The bars represent pixel volume means ± S.D. of the scanned bands on the immunoblot in arbitrary units.

    Article Snippet: Transferrin loading control was detected using rabbit anti-human transferrin polyclonal antibodies (1:1000, catalog number ADI-VAP-EN004-D, Enzo).

    Techniques: Western Blot, Affinity Purification, Clinical Proteomics, Control